By carefully diluting solutions and using spectrophotometric analysis, scientists can precisely determine concentrations. Why is serial dilution an important technique in laboratory science? If you begin with a 0. 5M solution and perform a 1:2 dilution three times, what will be the final concentration? How does a spectrophotometer measure concentration, and why do we use a blank solution? Predict how the absorbance. Diluting solutions is essential in the laboratory for using concentrated stock solutions. Prior to use, it's crucial to verify that Beer's Law applies to the species within the concentration range under investigation. A dilution can be performed not only to lower the concentration of the analyte that is being tested, so that it is in range, but also to help eliminate interferences from other substances that may be present in the sample that can artificially alter the analysis. In physics, this law is referred to as the Bouguer-Lambert law and. Reagent dilutions are needed daily in biochemical or immunological lab work.
[PDF Version]